Clone Assay:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Synthesized:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Expressing:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Plasmid Preparation:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Western Blot:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Sterility:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Cell Culture:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Sequencing:Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
Article Title: Proteomic detection and validation of translated small open reading frames.
Article Snippet: .. Materials: HEK293T cells (ATCC® CRL-3216™) HEK293T complete growth medium (See Recipe) Full length cDNA (obtained by cloning or synthesized by GenScript) pcDNA3.1 expression vector (Addgene, V790–20) Restriction enzymes (New England Biolabs) Lipofectamine 2000 (Invitrogen, 11668030) Opti-MEM Reduced Serum Media, (Thermo Fisher, 31985062) Precision Plus Dual Xtra Prestained Protein Standard (Bio-Rad, #1610377) 16% Tricine gels (Thermo Fisher, EC6695BOX) 2X Tricine SDS sample buffer (Invitrogen, LC1676) 10X Tricine SDS running buffer (Invitrogen, LC1675) Anti-FLAG M2 antibody (Sigma Aldrich, F3165) Rabbit anti-mouse HRP-conjugate (Abcam, ab97046) 10X SDS Running Buffer (Rockland, MB-017) 10X Transfer Buffer (VWR, 10128–706) Western ECL Substrate (Bio-Rad, 1705060S) Steri Cycle 37 °C, CO 2 Incubator (Thermo Fisher, TH-370N) Sterile Vacuum Filter Units (MilliporeSigma, SCGVU02RE) Sterile 1.5 mL plastic tubes Six-well plates (treated for cell culture) Gel tank and power supply adapters (Thermo Fisher, A25977) Power supply (Thermo Fisher, EC300XL2) Rocking platform Nitrocellulose and filter paper (Invitrogen, LC2000) Mini Blot Module for Western blot transfers (Invitrogen, B1000) Order cDNA clone synthesized by Genscript that represents the sequence of the RNA transcript to which your tryptic peptide of interest has been mapped. ..
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